Lysosomal Proteases Are a Determinant of Coronavirus Tropism
Identifieur interne : 000C66 ( Main/Exploration ); précédent : 000C65; suivant : 000C67Lysosomal Proteases Are a Determinant of Coronavirus Tropism
Auteurs : Yuan Zheng [États-Unis] ; Jian Shang [États-Unis] ; Yang Yang [États-Unis] ; Chang Liu [États-Unis] ; Yushun Wan [États-Unis] ; Qibin Geng [États-Unis] ; Michelle Wang [États-Unis] ; Ralph Baric [États-Unis] ; Fang Li [États-Unis]Source :
- Journal of Virology [ 0022-538X ] ; 2018.
Descripteurs français
- KwdFr :
- Animaux, Cellules A549, Cellules HEK293, Cellules HeLa, Cellules Vero, Cellules cultivées, Chiroptera, Coronavirus du syndrome respiratoire du Moyen-Orient (métabolisme), Coronavirus du syndrome respiratoire du Moyen-Orient (physiologie), Glycoprotéine de spicule des coronavirus (métabolisme), Humains, Infections à coronavirus (métabolisme), Lysosomes (enzymologie), Peptide hydrolases (métabolisme), Pénétration virale, Tropisme viral, Virus du SRAS (métabolisme), Virus du SRAS (physiologie).
- MESH :
- enzymologie : Lysosomes.
- métabolisme : Coronavirus du syndrome respiratoire du Moyen-Orient, Glycoprotéine de spicule des coronavirus, Infections à coronavirus, Peptide hydrolases, Virus du SRAS.
- physiologie : Coronavirus du syndrome respiratoire du Moyen-Orient, Virus du SRAS.
- Animaux, Cellules A549, Cellules HEK293, Cellules HeLa, Cellules Vero, Cellules cultivées, Chiroptera, Humains, Pénétration virale, Tropisme viral.
English descriptors
- KwdEn :
- A549 Cells, Animals, Cells, Cultured, Chiroptera, Chlorocebus aethiops, Coronavirus Infections (metabolism), HEK293 Cells, HeLa Cells, Humans, Lysosomes (enzymology), Middle East Respiratory Syndrome Coronavirus (metabolism), Middle East Respiratory Syndrome Coronavirus (physiology), Peptide Hydrolases (metabolism), SARS Virus (metabolism), SARS Virus (physiology), Spike Glycoprotein, Coronavirus (metabolism), Vero Cells, Viral Tropism, Virus Internalization.
- MESH :
- chemical , metabolism : Peptide Hydrolases, Spike Glycoprotein, Coronavirus.
- enzymology : Lysosomes.
- metabolism : Coronavirus Infections, Middle East Respiratory Syndrome Coronavirus, SARS Virus.
- physiology : Middle East Respiratory Syndrome Coronavirus, SARS Virus.
- A549 Cells, Animals, Cells, Cultured, Chiroptera, Chlorocebus aethiops, HEK293 Cells, HeLa Cells, Humans, Vero Cells, Viral Tropism, Virus Internalization.
Abstract
Coronaviruses are capable of colonizing new species, as evidenced by the recent emergence of SARS and MERS coronaviruses; they can also infect multiple tissues in the same species. Lysosomal proteases play critical roles in coronavirus entry by cleaving coronavirus surface spike proteins and activating the fusion of host and viral membranes; they also play critical roles in cell physiology by processing cellular products. How do different lysosomal protease activities from different cells impact coronavirus entry? Here, we controlled the contributions from known factors that function in coronavirus entry so that lysosomal protease activities became the only or the main determinant of coronavirus entry. Using pseudovirus entry, cell-cell fusion, and biochemical assays, we showed that lysosomal proteases from bat cells activate coronavirus spike-mediated membrane fusion more efficiently than their counterparts from human cells. Our study provides the first direct evidence supporting lysosomal proteases as a determinant of the species and tissue tropisms of coronaviruses.
Url:
DOI: 10.1128/JVI.01504-18
PubMed: 30258004
PubMed Central: 6258935
Affiliations:
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of Minnesota, Saint Paul, Minnesota, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota</wicri:regionArea><placeName><region type="state">Minnesota</region></placeName></affiliation></author><author><name sortKey="Wang, Michelle" sort="Wang, Michelle" uniqKey="Wang M" first="Michelle" last="Wang">Michelle Wang</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota</wicri:regionArea><placeName><region 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of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota</wicri:regionArea><placeName><region type="state">Minnesota</region></placeName></affiliation></author></analytic><series><title level="j">Journal of Virology</title><idno type="ISSN">0022-538X</idno><idno type="eISSN">1098-5514</idno><imprint><date when="2018">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>A549 Cells</term><term>Animals</term><term>Cells, Cultured</term><term>Chiroptera</term><term>Chlorocebus aethiops</term><term>Coronavirus Infections (metabolism)</term><term>HEK293 Cells</term><term>HeLa Cells</term><term>Humans</term><term>Lysosomes (enzymology)</term><term>Middle East Respiratory Syndrome Coronavirus (metabolism)</term><term>Middle East Respiratory Syndrome Coronavirus (physiology)</term><term>Peptide Hydrolases (metabolism)</term><term>SARS Virus (metabolism)</term><term>SARS Virus (physiology)</term><term>Spike Glycoprotein, Coronavirus (metabolism)</term><term>Vero Cells</term><term>Viral Tropism</term><term>Virus Internalization</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules A549</term><term>Cellules HEK293</term><term>Cellules HeLa</term><term>Cellules Vero</term><term>Cellules cultivées</term><term>Chiroptera</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (métabolisme)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (physiologie)</term><term>Glycoprotéine de spicule des coronavirus (métabolisme)</term><term>Humains</term><term>Infections à coronavirus (métabolisme)</term><term>Lysosomes (enzymologie)</term><term>Peptide hydrolases (métabolisme)</term><term>Pénétration virale</term><term>Tropisme viral</term><term>Virus du SRAS (métabolisme)</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Peptide Hydrolases</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Lysosomes</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Lysosomes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Coronavirus Infections</term><term>Middle East Respiratory Syndrome Coronavirus</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Glycoprotéine de spicule des coronavirus</term><term>Infections à coronavirus</term><term>Peptide hydrolases</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>A549 Cells</term><term>Animals</term><term>Cells, Cultured</term><term>Chiroptera</term><term>Chlorocebus aethiops</term><term>HEK293 Cells</term><term>HeLa Cells</term><term>Humans</term><term>Vero Cells</term><term>Viral Tropism</term><term>Virus Internalization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules A549</term><term>Cellules HEK293</term><term>Cellules HeLa</term><term>Cellules Vero</term><term>Cellules cultivées</term><term>Chiroptera</term><term>Humains</term><term>Pénétration virale</term><term>Tropisme viral</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Coronaviruses are capable of colonizing new species, as evidenced by the recent emergence of SARS and MERS coronaviruses; they can also infect multiple tissues in the same species. Lysosomal proteases play critical roles in coronavirus entry by cleaving coronavirus surface spike proteins and activating the fusion of host and viral membranes; they also play critical roles in cell physiology by processing cellular products. How do different lysosomal protease activities from different cells impact coronavirus entry? Here, we controlled the contributions from known factors that function in coronavirus entry so that lysosomal protease activities became the only or the main determinant of coronavirus entry. Using pseudovirus entry, cell-cell fusion, and biochemical assays, we showed that lysosomal proteases from bat cells activate coronavirus spike-mediated membrane fusion more efficiently than their counterparts from human cells. Our study provides the first direct evidence supporting lysosomal proteases as a determinant of the species and tissue tropisms of coronaviruses.</p></div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Caroline du Nord</li><li>Minnesota</li></region></list><tree><country name="États-Unis"><region name="Minnesota"><name sortKey="Zheng, Yuan" sort="Zheng, Yuan" uniqKey="Zheng Y" first="Yuan" last="Zheng">Yuan Zheng</name></region><name sortKey="Baric, Ralph" sort="Baric, Ralph" uniqKey="Baric R" first="Ralph" last="Baric">Ralph Baric</name><name sortKey="Geng, Qibin" sort="Geng, Qibin" uniqKey="Geng Q" first="Qibin" last="Geng">Qibin Geng</name><name sortKey="Li, Fang" sort="Li, Fang" uniqKey="Li F" first="Fang" last="Li">Fang Li</name><name sortKey="Liu, Chang" sort="Liu, Chang" uniqKey="Liu C" first="Chang" last="Liu">Chang Liu</name><name sortKey="Shang, Jian" sort="Shang, Jian" uniqKey="Shang J" first="Jian" last="Shang">Jian Shang</name><name sortKey="Wan, Yushun" sort="Wan, Yushun" uniqKey="Wan Y" first="Yushun" last="Wan">Yushun Wan</name><name sortKey="Wang, Michelle" sort="Wang, Michelle" uniqKey="Wang M" first="Michelle" last="Wang">Michelle Wang</name><name sortKey="Yang, Yang" sort="Yang, Yang" uniqKey="Yang Y" first="Yang" last="Yang">Yang Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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